Part:BBa_K3182010

Parseq-β

Parseq Beta is a de novo antimicrobial peptide. It was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010. This part was first ordered chemically synthesised by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea due to their hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria was combined with the antimicrobial peptides in different concentrations. They were then incubated at 37°C, with shaking, every 15 minutes. The plate was incubated for 16 hours and OD⁶⁰⁰ was measured. The results can be seen in Figure 1..

Figure 1. Shows the antimicrobial activity of Parseq Beta on E.Coli. The OD⁶⁰⁰ value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. NG stands for no growth and MIC stands for minimum inhibitory concentration. The positive control was E.coli cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.

Figure 2. Shows the antimicrobial activity of Parseq Beta on B.Subtilis. The OD⁶⁰⁰ value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. The positive control was B.Subtilis cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]