Composite

Part:BBa_K3102026:Design

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 17:49, 13 October 2019 by SDUQUESNE (Talk | contribs) (→‎References)

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T7-RBS-LDHA-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 489
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 219


Design Notes

None


Source

E.coli, sequence found through Ebi https://www.ebi.ac.uk/ena/data/view/AAC74462

Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Bunch PK, Mat-Jan F, Lee N, Clark DP. "The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli". Microbiology. 1997;143(1 997):187–95.