Terminator

Part:BBa_K3105676:Design

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-11)
Revision as of 17:36, 18 October 2019 by JonasG (Talk | contribs)


pPICZαB fragment + AOX1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 131
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Functionality in Pichia pastoris
A double stop codon is introduced in the 5'end to stop protein translation. Residues of the pPICZαB vector are present before the actual terminator starts.


Source

Organism : Pichia pastoris
DNA originates from the pPICZαB vector found in the invitrogen easy select Pichia expression kit

References