Coding

Part:BBa_K3282002

Designed by: Manjyot Kaur, Mateusz Piotr Szewczyk   Group: iGEM19_Lund   (2019-10-11)
Revision as of 18:45, 11 October 2019 by ParselTMatts (Talk | contribs)


C. metallidurans lead binding protein - pbrD

This part contains coding sequence of Cupriavidus metallidurans CH34 lead binding protein (pbrD, Gene ID: 3170401, Uniprot: Q58AJ8) codon-optimized for Escherichia coli Nissle 1917. Pb(II) resistance was early recognized to be located on pMOL30, one of the two plasmids found in Cupriavidus metallidurans CH34 (1). The pbrD gene encodes a Pb(II)-binding protein which is essential for functional lead sequestration. When Pb(II) enters the cytoplasm, it can be bound by the PbrD protein, which may function as a chaperone for Pb(II) and cells lacking PbrD show a decreased accumulation of Pb(II) and this accumulation may protect against free exported Pb(II)(2).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None