Coding

Part:BBa_K3076300:Design

Designed by: Choi Jonas Sze Ngo   Group: iGEM19_Hong_Kong_JSS   (2019-10-05)
Revision as of 13:12, 13 October 2019 by Pok430 (Talk | contribs)


dsDNA substrate with KanR gene for CopA knockout in E. coli by Lambda Red Recombineering


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The genomic sequence of CopA gene was extracted from NCBI (ACCESSION: NC_000913 REGION: complement (508875..511379)). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence.

Design Notes

The recombination site was chosen to be near the C terminal of the gene to increase the chance of successful knockout.

References