Part:BBa_K2968002
sRNA GcvB targeting BBa_B0034 and BBa_E0040 start codon
This sRNA has promoter target binding region for BBa_B0034 and BBa_E0040 start codon
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
KCL iGEM 2019
BBa_K2968002
To generate functional sRNA molecule with the secondary structure shown in figure 1 our team has created this composite part consisting of three basic parts: BBa_J23100 promoter, BBa_K2968014 mRNA target binding region and BBa_K2968012 GcvB sRNA scaffold as shown in figure 2.
Figure 1. BBa_K2968002 expressing sRNA secondary structure predicted using http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi server. The mRNA target binding region is highlighted in pink and the sRNA scaffold in teal.
Figure 2. Details of the BBa_K2968002 sRNA construct design
The BBa_J23100 promoter is a strong promoter from the constitutive promoter family designed by John Anderson, iGEM2006_Berkeley. BBa_K2968014 part is a complementary part to the BBa_K608010 that has BBa_B0034 Ribosome Binding Site (RBS) and BBa_E0040 GFP gene part as shown in figure 3. The GcvB scaffold is the 84 bp region of the E.coli sRNA.
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