Part:BBa_K3058002
Uric acid-induced Uricase generator
This device could produce uricase only when there is uric acid. In the device, HucR is a repressor than can bind to HucO operator to inhibit the gene expression down stream HucO. In the presence of uric acid, the structure of HucR is altered and is no longer able to bind HucO, leading to the down stream gene expression. We inserted a uricase gene (URI) downstram HucO. In this case, uricase is expressed only when there is significant level of uric acid. To better visualize the gene expression, we include RFP(E1010) downstream HucR and a GFP(E0040) downstream URI. In our project, we express it in E. coli. It is hoped that the uricase-expressing E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2832
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2362
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2832
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2832
Illegal NgoMIV site found at 867
Illegal AgeI site found at 156
Illegal AgeI site found at 268
Illegal AgeI site found at 2384 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3498
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