Coding
ADR CMYC 6

Part:BBa_K3038001:Experience

Designed by: Marine LAVAUD   Group: iGEM19_Poitiers   (2019-10-10)
Revision as of 09:15, 10 October 2019 by TonyH (Talk | contribs) (→‎Applications of BBa_K3038001)


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Applications of BBa_K3038001

Characterization of the ADR constructs:


Electrophoresis gel photography following deposits of N-ter and C-ter ADR PCR products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is NEB 1 kb Plus DNA Ladder.



Modelization of the ligation between ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.

File:Images/5/5b/T--Poitiers--plasmid construction ADR-tab3.jpg
Figure 1. Single stranded DNA harvested from phage and run on a gel. The higher bands are phage genomic DNA while the lower bands at roughly 2-3kb correspond to the expected plasmid size.

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