Device
Cry4Ba Pla

Part:BBa_K2938014

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-10-09)
Revision as of 09:16, 10 October 2019 by Assafi (Talk | contribs)


Cry4Ba (HA tag) + deGFP Device

PR1 - RBS - Cry4Ba (HA tag) - RBS - deGFP - T500 Terminator

This plasmid was planned on a pBEST plasmid backbone. It contains larvicidal activity, as it containing toxic subunit of the bacillus thuringiensis israelensis (BTI) pBtoxis plasmid which is toxic to mosquitoes larva.

The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1859
    Illegal XbaI site found at 3597
    Illegal SpeI site found at 2548
    Illegal PstI site found at 363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
    Illegal NheI site found at 105
    Illegal NheI site found at 247
    Illegal SpeI site found at 2548
    Illegal PstI site found at 363
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4330
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1859
    Illegal XbaI site found at 3597
    Illegal SpeI site found at 2548
    Illegal PstI site found at 363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1859
    Illegal XbaI site found at 3597
    Illegal SpeI site found at 2548
    Illegal PstI site found at 363
    Illegal NgoMIV site found at 2671
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 653


[edit]
Categories
Parameters
None