Composite

Part:BBa_K3102032

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 13:21, 14 October 2019 by ChloeDOIZELET (Talk | contribs)

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T7-RBS-ZWF-Ter

This T7-RBS-ZWF-ter composite part is made of the glucose-6-phosphate 1-dehydrogenase enzyme which catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone in the pentose phosphate pathway. Due to the knock-out of PGI, there is a metabolic flux shift toward the pentose phosphate shunt using this enzyme to allow the bacteria to survive. This part is useful to increase flavin production.

This construct is enriched with T7 promoter, RBS and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 810
    Illegal NotI site found at 691
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 810
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 810
    Illegal NgoMIV site found at 83
    Illegal NgoMIV site found at 800
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None