Composite

Part:BBa_K3102027:Design

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 09:15, 12 October 2019 by SDUQUESNE (Talk | contribs) (→‎References)


T7-RBS-LLDP-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 324
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 460
    Illegal AgeI site found at 923
    Illegal AgeI site found at 1361
    Illegal AgeI site found at 1520
    Illegal AgeI site found at 1532
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

None

References

Dong JM et al., Three overlapping lct genes involved in L-lactate utilization by Escherichia coli., Journal of Bacteriology, 1993 Oct;175(20):6671-8.

Nunez MF et al., Transport of L-Lactate, D-Lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli, Biochem Biophys Res Commun. 2002 Jan 18;290(2):824-9.