Reporter

Part:BBa_K2986004

Designed by: zheng shuxin   Group: iGEM19_SUSTech_Shenzhen   (2019-10-07)
Revision as of 07:29, 7 October 2019 by Susan1999 (Talk | contribs)


hGluc

The luciferase from the marine copepod Gaussia princeps (Gluc) is the smallest (∼19.9-kDa) coelenterazine (CTZ)-utilizing luciferase known thus far. A humanized variant of Gluc (hGluc), which was codon optimized for expression in cultured mammalian cells, has been widely used as a reporter gene. Gaussia luciferase exhibits an activity up to 1,000-fold higher than to Renilla reniformis luciferase (Rluc), firefly luciferase (Fluc), or bacterial luciferases (LuxAB). The outstanding sensitivity of Gluc-based assays was previously demonstrated detecting as low as 10−18 mol purified Gluc or one eukaryotic cell transiently expressing Gluc.

The plasmid with il8 and hgluc.png


Design Notes

how to use the hGluc to represent the cytokine expression.


Source

Snapgene

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 604
[edit]
Categories
Parameters
None