Scar

Part:BBa_K2996602

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-05)
Revision as of 10:43, 7 October 2019 by Pedestal (Talk | contribs)


scar between RSRL and EGFP

After constructing pGEX4t-EGFP, we gave it to company to synthesize our desired RSRL sequence. Since the fragment can only be put between restrcition sites, there is a scar left between RSRL and EGFP. Actually, it is the restrcition site for BamHⅠ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
Figure 1. 33333. 4567876543


[edit]
Categories
Parameters
None