Part:BBa_K2920001

Peptide production process

-BBa_I719005 (T7 Promoter) Constitutive promoter derived from the T7 bacteriophage. Allows high expression of proteins only when the T7 polymerase is present.

-BBa_K128005 (His affinity tag) This codes for six Histidine residues to allow for His column protein purification. It will work best if put at either the N or C terminus of your protein construct.

-BBa_K2323012 (SUMO tag) The SUMO tag increases solubility of the tagged protein and can be cleaved with the SUMO protease.

-BBa_K1911005(Egfp) Enhanced green fluorescent protein which can be used as a reporter gene. eGFP can be used as a reporter gene and helps the observer easily see the coloration of E. coli cells which indicate a successful transformation.

-BBa_K731721(T7 terminator) wild type terminator from T7 bacteriophage.

We used the different functions of the biobrick to increase the process efficiency. For example, the SUMO can help the peptide increases the solubility, the egfp protein can helps the observer easily see the coloration of E. coli , and the his tag will help us with the protein purification.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 362
  Illegal NheI site found at 1904
  Illegal NheI site found at 2664
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1570
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2447
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1005