Composite

Part:BBa_K3111202

Designed by: Matas Deveikis   Group: iGEM19_UCL   (2019-09-18)
Revision as of 15:21, 5 October 2019 by Matas deveikis (Talk | contribs) (→‎Experimental Results)


DARPin929_mScarlet_StrepII

This part encodes DARPin929_mScarlet. The use of this part enabled us to study the binding and uptake of DARPin929 by conducting cell culture studies on SK-BR-3 HER2+ cells. The DARPin is flanked by red fluorescent protein mScarlet on the C-terminus which allowed visualisation of binding under confocal microscopy and a StrepII-tag downstream of this fusion, which allows for column-based purification of the expressed protein. It is expressed under a T7 promoter and a strong RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 575
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1147
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Results

DNA Analysis

Protein analysis

Binding to HER2 receptor

Conclusion

[edit]
Categories
Parameters
None