Coding

Part:BBa_K3076100:Design

Designed by: Choi Jonas Sze Ngo   Group: iGEM19_Hong_Kong_JSS   (2019-10-04)
Revision as of 14:34, 5 October 2019 by Jonaschoi1028 (Talk | contribs)


Protein coding sequence for Saccharomyces cerevisiae metallothionein 1 (CUP1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains the genomic sequence of CgMT. It was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906) and then codon-optimized for E. coli by ThermoFisher GeneArt platform.

References

[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064