Part:BBa_K2996003
Tac promoter and operator plus RBS
Tac-Promoter (abbreviated as Ptac) is a synthetically produced DNA promoter, produced from the combination of promoters from the trp and lac operons. It is commonly used for protein production in Escherichia coli
We constructed the pRead plasmid for information storage, where RSRL array and EGFP are fused downstream of this promotor. Spacer acquisition carried out by cas1/2 would cause an addition of 61 base pairs, a protospacer and another repeat sequence, thus resulting in frame shifting. In that case, the stop codon would disappear, and EGFP enter the correct reading frame.
Whenever you want to read the information, add IPTG to a final concentration of 0.5M and incubate at 20℃, leading to the expression of fluorescent protein.
For more detailed results, click BBa_K2996002.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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