Composite

Part:BBa_K3221008:Design

Designed by: Qihan Jin   Group: iGEM19_SCU-China   (2019-10-04)
Revision as of 09:51, 4 October 2019 by Jinqihan (Talk | contribs) (→‎Source)

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pGAL1-HisG only-tCYC1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use double enzymes restriction of HindIII and XbaI before adapter ligation.

Source

pGAL1 and tCYC1 is on pYES2 initially. And we get HisG-only by PCR from CNS3(BBa_K3221001).

References