Part:BBa_K3221201

MET3 promoter(pMET3) is a tight promoter which comes from Saccharomyces cerevisiae BY4742. The pMET3 regulates MET3 gene expression, which is a vital enzyme in the methionine biosynthetic pathway in Saccharomyces cerevisiae. This promoter can be regulated by methionine. The high concentration of methionine can inhibit the promoter’ s expression, and it can turn on when the methionine in the medium at a low concentration. Because of this characteristic, we used it in our delay expression system.