Regulatory
Part:BBa_K2967021:Design
Designed by: Lijun Bao Group: iGEM19_NEU_CHINA (2019-10-02)
PytfE promoter, responsive to nitrate, nitrite and nitric oxide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part can be used to detect the nitric oxide.
Source
The Escherichia coli NsrR protein is a nitric oxide‐sensitive repressor of transcription.Reference:Partridge, J.D., et al., NsrR targets in the Escherichia coli genome: new insights into DNA sequence requirements for binding and a role for NsrR in the regulation of motility. Mol Microbiol, 2009. 73(4): p. 680-94.