Part:BBa_K3098024
GxCesA
Free radicals, such as superoxide anions (O2·-) or hydroxyl radicals (HO·), can cause protein damage and DNA mutations that can initiate several diseases, including coronary heart diseases and diabetes. Antioxidants that can scavenge free radicals play an important role in biological systems.An antioxidant peptide derived from Pinctada fucata has great poteintial for scavenging free radicals. Howeverthe the low rates of enzymatic hydrolysis extraction and purification, as well as the higher costs for purifying from Pinctada fucata meat, suggest that this natural and safe antioxidant peptide would not meet industrialized production requirements. Thus we submit a designed AOP that can be easily produced and purified in E.coli, named 'DSAOP'.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 580
Illegal PstI site found at 742 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 580
Illegal PstI site found at 742 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 580
Illegal BglII site found at 1142
Illegal XhoI site found at 232 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 580
Illegal PstI site found at 742 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 580
Illegal PstI site found at 742
Illegal NgoMIV site found at 212
Illegal AgeI site found at 2193 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1717
chassis | E.coli BL21 |