Coding
DSAOP

Part:BBa_K3098024

Designed by: Yiru Shen   Group: iGEM19_WHU-China   (2019-10-01)
Revision as of 04:55, 2 October 2019 by RiruShen (Talk | contribs) (DSAOP(Dedigned Antioxidant peptides))


GxCesA

Free radicals, such as superoxide anions (O2·-) or hydroxyl radicals (HO·), can cause protein damage and DNA mutations that can initiate several diseases, including coronary heart diseases and diabetes. Antioxidants that can scavenge free radicals play an important role in biological systems.An antioxidant peptide derived from Pinctada fucata has great poteintial for scavenging free radicals. Howeverthe the low rates of enzymatic hydrolysis extraction and purification, as well as the higher costs for purifying from Pinctada fucata meat, suggest that this natural and safe antioxidant peptide would not meet industrialized production requirements. Thus we submit a designed AOP that can be easily produced and purified in E.coli, named 'DSAOP'.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 580
    Illegal PstI site found at 742
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 580
    Illegal PstI site found at 742
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 580
    Illegal BglII site found at 1142
    Illegal XhoI site found at 232
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 580
    Illegal PstI site found at 742
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 580
    Illegal PstI site found at 742
    Illegal NgoMIV site found at 212
    Illegal AgeI site found at 2193
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1717


[edit]
Categories
Parameters
chassisE.coli BL21