Coding

Part:BBa_K3221001:Design

Designed by: Qihan Jin   Group: iGEM19_SCU-China   (2019-10-01)
Revision as of 06:34, 1 October 2019 by Jinqihan (Talk | contribs) (→‎References)

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CNS3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2614
    Illegal SpeI site found at 1275
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1303
    Illegal SpeI site found at 1275
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1747
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2614
    Illegal SpeI site found at 1275
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2614
    Illegal SpeI site found at 1275
    Illegal AgeI site found at 2677
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are two domains in this parts, NK and HisG. They are relatively independent, so we can devide them by PCR.


Source

We got this part by gene synthesis. And it can also be found in the genome of Cordyceps militaris.

References

Xia Y , Luo F , Shang Y , et al. Fungal Cordycepin Biosynthesis Is Coupled with the Production of the Safeguard Molecule Pentostatin[J]. Cell Chemical Biology, 2017:S2451945617303276.