Composite
COMP

Part:BBa_K3128033:Experience

Designed by: Fahd TIBOURTINE   Group: iGEM19_Grenoble-Alpes   (2019-09-29)
Revision as of 20:21, 11 October 2019 by Fahd (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3128033

<Click-iT ™ Alexa Fluor ™ 488 sDIBO Alkyne confirmation:> Click-iT ™ Alexa Fluor ™ 488 sDIBO alkyne was used to confirm whether Ompx is in memebrane and if the unnatural amino acid is incorporated into OmpX. This fluorophore is used to check the reaction of the click. If the unnatural amino acid is present, the fluorophore should "click" on the OmpX transmembrane protein and remain there. This can be analyzed with fluorescence microscopy Here are the results obtained on unprocessed BL21 (1) and the results obtained for BL21 cotransformed with the 2 vectors (2).

User Reviews

UNIQc18ec57f1cc124d2-partinfo-00000000-QINU UNIQc18ec57f1cc124d2-partinfo-00000001-QINU