Coding
COMP
Part:BBa_K3128032
Designed by: Fahd TIBOURTINE Group: iGEM19_Grenoble-Alpes (2019-09-29)
COMP : Clickable OmpX
Clickable OmpX is an outer membrane protein whose C and N terminal in the intracellular domain. It can be used as a scaffold if an unnatural amino acid is to be introduced. This can be done through the presence of the amber stop codon TAG in the third loop OmpX. With a specific tRNA, an amino acid functionalized with an azide will be integrated, which can be used for the SPAAC click chemistry reaction with DBCO / DIBO functional groups. Once expressed, this biobrick will serve as a positive control to check the click for biobrick BBa_K3128015 et BBa_3128016 during the expression test
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |