Composite

Part:BBa_K3064016:Design

Designed by: Jie Cai   Group: iGEM19_NUDT_CHINA   (2019-09-26)
Revision as of 11:43, 20 October 2019 by Caijie (Talk | contribs)

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mmuIgG-Fc->HA-Trim21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 646
    Illegal BamHI site found at 1349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 747
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7


Design Notes

We disassemble the sequence of IgG and retain the Fc domain. We get the sequence of Trim21 and add the HA tag.

Source

BBa_K3064009, BBa_K2653004, BBa_K3064010