Coding

Part:BBa_K3064009

Designed by: Jie Cai   Group: iGEM19_NUDT_CHINA   (2019-09-26)
Revision as of 09:06, 20 October 2019 by Caijie (Talk | contribs)


mmuIgG-Fc

Immunoglobulin G (IgG) is a type of antibody, containing the fragment crystallizable region(Fc).

Usage and Biology

As a type of antibody, IgG binds many kinds of pathogens and protects the body from infection. IgG shapes like a Y due to its tetrameric quaternary structure, with two identical class heavy chains and two identical light chains. On the bottom of the heavy chain is the Fc domain. Fc mediates different physiological effects of antibodies by interacting with various cell receptors and complement proteins. Also, it can be recognized and bound with TRIM21,an E3 ubiquitin-protein ligase that has been applied to a protein degradation method known as Trim-Away.

In our project, this part is linked with glucagon. Glucagon binds to glucagon receptor(GCGR), Fc domain recruits the TRIM21, leading to the ubiquitination and final degradation of GCGR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7

References

1.IgG http://wikipedia.moesalih.com/IgG

2.Fc http://wikipedia.moesalih.com/Fragment_crystallizable_region


[edit]
Categories
//collections/immune_regulation/antibodies
Parameters
None