Coding

Part:BBa_K2973009:Experience

Designed by: Nick Delkis, Aphrodite Katsaouni   Group: iGEM19_Thessaly   (2019-08-26)
Revision as of 20:02, 28 September 2019 by Nickdelkis (Talk | contribs) (→‎Applications of BBa_K2973009)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2973009

In our project, we used the IS6110 gene as a DNA template, in order to perform isothermal amplification reactions with Recombinase Polymerase Amplification (RPA). Using this method, we were able to detect quantities as low as 10 to the minus 9 nanograms in reaction times ranging from five to 20 minutes. The primers used in the amplification reactions were BBa_K2973016 (forward) and BBa_K2973017 (reverse). These primers include 5' overhangs ( a T7 promoter and a trigger sequence) to allow for transcription by a T7 RNA Polymerase and translation by a toehold-switch regulated system encoding for a reporter gene (BBa_K2973007).

RPA reaction after clean up of the amplified product

HTML img Tag

PCR reaction

HTML img Tag

User Reviews

UNIQ2d975825a6dc2fc0-partinfo-00000002-QINU UNIQ2d975825a6dc2fc0-partinfo-00000003-QINU