Primer

Part:BBa_K2973016:Design

Designed by: Nick Delkis, Aphrodite Katsaouni   Group: iGEM19_Thessaly   (2019-09-28)
Revision as of 19:45, 28 September 2019 by Nickdelkis (Talk | contribs) (→‎References)


IS6110 Forward Primer with 5' Overhang


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primer design was accomplished using the Primer3 software (Primer-BLAST). The T7 Promoter on the 5' end of the sequence allows for downstream transcription of the amplified product by a T7 RNA Polymerase.


Source

iGEM Part Registry

References

https://www.ncbi.nlm.nih.gov/tools/primer-blast/