Composite
Part:BBa_K2973014:Design
Designed by: Thodoris Kontogiannis Group: iGEM19_Thessaly (2019-09-28)
Trigger for Toehold switch 14 Dictyoglomus turgidum
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 64
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This composite part was designed in order to achieve the transcription of a small part of the 16S rRNA gene of Dictyoglomus kaustophilus. This part behaves as a trigger sequence that will activate the Toehold switch 14 (BBa_K2973012).
Source
Dictyoglomus turgidum strain DSM 6724 16S ribosomal RNA, partial sequence
NCBI Reference Sequence: NR_043385.1