Part:BBa_K2938007
Cyt1Aa With Strep Tag
Cyt1Aa Bti toxin with Strep-tag attached in the C- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)
Description
Pesticidal crystal protein Cyt1Aa is a 27 kDa protein which can be isolated from Bacillus thuringiensis subsp. israelensis (Bti). The pBtoxis is a toxin-encoding plasmid that its origin is the Bti and it encodes to four Cry toxins (Cry4Aa, Cry4Ba, Cry10Aa and Cry11Aa) and three Cyt toxins (Cyt1Aa, Cyt2Ba and Cyt1Ca)[1], which are specifically toxic against Aedes, Anopheles and Culex mosquitoes which are vectors to many diseases caused by parasites and viruses, such as Malaria, Zika and Dengue. Each of the toxins hold a low level of larvicidal effect but their combination has a synergetic effect which is stronger than the linear combination we expect. The Cyt1Aa toxin effect in the synergetic effect is mainly the prevention of resistance in the mosquito to the Cry toxins. The bigger Cry toxins, Cry4Aa and Cry4Ba have a conserved C-terminal end which helps the toxin fold correctly and form crystal structures.
Amplification
Usage and Biology
The Cyt1Aa is a toxin with larvicidal effect on mosquitoes larvae, and strep-tag is used to detect proteins in western blot using antibodies specific to the tag.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 419
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 419
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 717
Illegal BamHI site found at 96 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 419
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 419
- 1000COMPATIBLE WITH RFC[1000]
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