Composite

Part:BBa_K3044006

Designed by: Anne Sofie Løgstrup Henriksen   Group: iGEM19_SDU-Denmark   (2019-09-27)
Revision as of 00:58, 22 October 2019 by Annemette (Talk | contribs)

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Strong constitutively active Anderson promoter (0.86) with a GFP coding sequence

This part make bacteria express the GFP protein. The SDU-Denmark 2019 team used this part to create an E. coli that expressed GFP. A plasmid containing a (d)Cas9/sgRNA system were transformed into this chassis, and by fluorescence microscopy and FACS it was validated whether the (d)Cas9/sgRNA could downregulate the expression of GFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


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Categories
Parameters
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