Composite
Part:BBa_K3044006
Designed by: Anne Sofie Løgstrup Henriksen Group: iGEM19_SDU-Denmark (2019-09-27)
Strong constitutively active Anderson promoter (0.86) with a GFP coding sequence
This part make bacteria express the GFP protein. The SDU-Denmark 2019 team used this part to create an E. coli that expressed GFP. A plasmid containing a (d)Cas9/sgRNA system were transformed into this chassis, and by fluorescence microscopy and FACS it was validated whether the (d)Cas9/sgRNA could downregulate the expression of GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
[edit]
Categories
Parameters
None |