Coding

Part:BBa_K3113102:Design

Designed by: Theresa Keil   Group: iGEM19_Munich   (2019-09-26)
Revision as of 08:40, 26 September 2019 by Theresakeil (Talk | contribs) (→‎Source)


P9SN


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloning Primers: fw AATTCGTGAGCGGCTGGCGGCTGTTCAAGAAGATTAGCGGCGGGAGTGCTAGCAGCCCGGAAGATGAAAACCAGAGCCTGGAACAGAAAAACAGCCAGCTGAAACAGGAAATTAGCCAGCTGGAACAGGAAATTCAGCAGCTGGAATATTGAA rev CGCGTTCAATATTCCAGCTGCTGAATTTCCTGTTCCAGCTGGCTAATTTCCTGTTTCAGCTGGCTGTTTTTCTGTTCCAGGCTCTGGTTTTCATCTTCCGGGCTGCTAGCACTCCCGCCGCTAATCTTCTTGAACAGCCGCCAGCCGCTCACG


Source

This sequence was provided by our supervisor PhD student Susanne Pettinger, Westmeyer Lab, Helmholtz Center Munich.

Ljubetič, A., et al. (2017). "Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo." Nature Biotechnology 35: 1094. https://www.nature.com/articles/nbt.3994#s1, Supplementary Text and Figures

References