Part:BBa_K2933008

subclass B1 metallo-beta-lactamase PEDO-1, codon optimized in E. coli

This part encodes a protein called PEDO-1, which is a metallo-beta-lactamase of subclass B1.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 537
  Illegal PstI site found at 823
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 537
  Illegal PstI site found at 823
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 537
  Illegal PstI site found at 823
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 537
  Illegal PstI site found at 823
  
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

blaPEDO-1, a new carbapenem-hydrolyzing beta-lactamases, this enzyme has not yet emerged in clinical settings but constitute potential carbap- enem resistance determinants in pathogenic bacterial species, as demonstrated by their ability to confer resistance to ampi- cillin and various cephalosporins, as well as reduced suscepti- bility to carbapenems, once expressed in E. coli.

Molecular cloning

First, we used the vector pGEX-6p-1 to construct our expression plasmid. And then we converted the plasmid constructed to E. coli DH5α to expand the plasmid largely.

Figure 1. Left: The PCR result of PEDO-1. Right: The verification results by enzyme digestion.