Part:BBa_K2957007
hEF1a, IL-8 (CXCL-8), NeonGreen Tag
Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance
What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression
What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.
How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1256
Illegal PstI site found at 699 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 699
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 473
Illegal BamHI site found at 715 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1256
Illegal PstI site found at 699 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1256
Illegal PstI site found at 699
Illegal AgeI site found at 81
Illegal AgeI site found at 1262 - 1000COMPATIBLE WITH RFC[1000]
None |