Coding
otsA

Part:BBa_K3125000:Design

Designed by:   Group: iGEM19_Tacoma_RAINmakers   (2019-09-22)
Revision as of 21:41, 22 September 2019 by Catherinelinnea (Talk | contribs) (→‎Design Notes)

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trehalose-6-phosphate synthase from Rhizobium etli CFN 42


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 284
    Illegal XhoI site found at 148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 373
    Illegal NgoMIV site found at 457
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 935
    Illegal NgoMIV site found at 1102
    Illegal NgoMIV site found at 1357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 727


Design Notes

[1]

Source

GenBank AY731700.1 with 6 silent mutations made to decrease GC content during DNA synthesis. 4 illegal PstI sites and 1 illegal EcoRI site removed

References

References: Iordachescu M. and Imai R. (2011). Trehalose and Abiotic Stress in Biological Systems, Abiotic Stress in Plants - Mechanisms and Adaptations, Prof. Arun Shanker (Ed.), ISBN: 978-953-307-394-1, InTech, Available from: http://www.intechopen.com/books/abiotic-stress-in-plants-mechanisms- andadaptations/trehalose-and-abiotic-stress-in-biological-systems Suarez R. Wong A. Ramirez M. Barraza A. Orozco M. D. C. Cevallos M. S. Lara M. Hernandez G. Iturriaga G. (2008) Improvement of drought tolerance and grain yield in common bean by overexpressing trehalose-6-phosphate synthase in rhizobia. Molecular Plant-Microbe Interactions, 21 7 (July 2008), 958 966 , 0894-0282 UNIPROT https://www.uniprot.org/uniprot/P31677. Accessed Sept 20, 2019.