Coding
P20

Part:BBa_K2938004

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-09-19)
Revision as of 10:30, 28 September 2019 by Assafi (Talk | contribs)


P20

Description

p20 is a 20 kDa protein which can be isolated from Bacillus thuringiensis subsp. israelensis (Bti). It is a chaperone, which increases the production of Cry11Aa while expressed, and leads to higher mosquito larvicidal activity [2].


Amplification

PCR amplification of P20 by PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 264
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 264
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 264
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 264
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None