Composite

Part:BBa_K3128024

Designed by: Melodie Dupre   Group: iGEM19_Grenoble-Alpes   (2019-09-18)
Revision as of 21:38, 18 September 2019 by Melo (Talk | contribs)


COMP fused with T18 subpart of Bordetella Pertussis AC under lactose promoter

This biobrick is composed of the external membrane OMPx protein with Y121F' mutation fused at its N-terminal end with a 54 aa GGS linker (BBa_K3128010) followed by the T18 subunit of the Bordetella Pertussis adenylate cyclase. This biobrick is under plac promoter in order to be activated when the cAMP messenger is present in the system.

This biobrick has a TAG codon (Y121F' mutation) which can be used to introduce any Non Natural Amino acid outside the cell (more informations:LIEN!!).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1601
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1142
    Illegal NgoMIV site found at 1552
    Illegal AgeI site found at 1358
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 344


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Categories
Parameters
None