Coding

Part:BBa_K2996703

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-09-16)
Revision as of 06:43, 6 October 2019 by Pedestal (Talk | contribs)


RpoA, DNA-directed RNA polymerase subunit alpha

RpoA encodes the DNA-directed RNA polymerase subunit alpha, which plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme. It may activate transcription more effectively, compared to RpoZ used in other expereiments.

This RpoA-dCas9 fusion protein should be an inovated transcription activator.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 459
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None