Part:BBa_K3022003
lac-CFppk1 Cassette
This part will be sub cloned into pSB1C3, a plasmid of high copy number, to yield pSB1C3-lac-CFppk1. Polyphosphate synthesis capability of C. freundii transformed with this plasmid will be subjected to compare with C. freundii that harbored medium-copy expressing vector pBBR1MCS2-CFppk1. In this way, we can demonstrate that why medium copy number is a better option of vector choice.
In addition of CFppk1, this part also includes the promoter and terminator sequences of the lac expression system, thus composing a CFppk1 expression cassette. As such, when it was sub cloned into a plasmid of certain copy number, we can get a relative precise result regarding plasmid copy-number’s effects on ppk1 expressing level and polyphosphate yields, rather than a promiscuous conclusion drawn from the differences of promoting and/or terminating strength imposed by differed plasmid expressing system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |