Coding

Part:BBa_K2992008

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-09)
Revision as of 14:37, 9 September 2019 by YasTen (Talk | contribs) (→‎Usage and Biology)


Thiolase gene thl from C. acetobutylicum.

The thiolase gene encodes an acetyl-coenzyme A acetyltransferases from C. acetobutylicum.


Usage and Biology

Thiolases are core metabolic enzymes involved in the melavonate pathway responsible for the conversion of acetyl-CoA to acetoacetyl-CoA. Results from our genome scale modeling suggested that exogenous thiolase was required for the production of acetone in our chosen volatile reporter organism C. sporogenes. Thiolase was therefore included in our acetone production pathway constructs in order to a volatile reporter for the prediction of botulinum toxin production following food manufacturing processes.

Characterisation

Data incoming.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 355
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 355
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 355
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Incoming.

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Categories
Parameters
None