Part:BBa_K2963004

racE - a glutamate racemase from Bacillus sp.

This part contains the racE gene for glutamate racemase from Bacillus. This enzyme mainly catelyse the reation of L-Glumate to D-Glumate.In our project, we express this enzyme simultaneously coexpressing PgsBCA complex in order to try to make poly-gamma-glutamic acid whose D-glutamate content is different.

Usage and Biology

The racE gene is derived from Bacillus subtilis and it encodes a racemase which can converts L-glutamate to D-glutamate.

Characterization

We used enzyme activity data to prove the usability of our racE. We measured the enzymatic activity under different tac promoters.

We tested the enzyme activity of the racemase in cells. After induction by IPTG, the results showed that the activity of racemase could be detected under the control of different tac promoters, indicating that this part could be used. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]