Coding

Part:BBa_K2963003

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-07)
Revision as of 10:37, 7 September 2019 by GHX (Talk | contribs)


capB* - a subunit of poly-γ-glutamic acid synthetase

This part contains the pgsB gene for poly-gamma-glutamic acid synthetase. This enzyme is involved in poly-gamma-glutamic acid synthesis,which converts the glutamate to poly-gamma-glutamic acid. PgsB is mainly responsible for catalysing poly-gamma-glutamic acid synthesis. In our subject, we get the pgsB sequenece by error-prone PCR of Bacillus genome, trying to improve the activity of PgsB protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 737
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 737
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 737
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 737
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 737
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None