Composite
Part:BBa_K2927064
Designed by: KUO, PEI-CHING Group: iGEM19_CCU_Taiwan (2019-09-04)
T7 promoter-guide RNA-crRNA 4-mutated crRNA 5
The sequence we designed is used for generating crRNA 4 and mutated crRNA 5. CrRNA 4 is used for African swine fever virus (ASFV) detection. Furthermore, mutated crRNA 5 is the sequence derived from crRNA 5 for negative control to test whether our purified Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 4 and mutated crRNA 5.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |