Composite

Part:BBa_K2927066

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-09-04)
Revision as of 14:41, 25 September 2019 by Peggy KUO (Talk | contribs)


T7 promoter-guide RNA-crRNA 6-mutated crRNA 3

The sequence we designed is used for generating crRNA 6 and mutated crRNA 3. CrRNA 6 is used for African swine fever (ASFV) detection. Furthermore, mutated crRNA 3 is the sequence derived from crRNA 3 for negative control to test whether our homemade Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 6 and mutated crRNA 3.

The sequence we designed is used for generating crRNA 6 and mutated crRNA 3. CrRNA 6 is used for African swine fever virus (ASFV) detection. Furthermore, mutated crRNA 3 is the sequence derived from crRNA 3 for negative control to test whether our purified Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 6 and mutated crRNA 3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None