Coding

Part:BBa_K3039008:Design

Designed by: Ceilidh Welsh   Group: iGEM19_Exeter   (2019-08-30)
Revision as of 09:52, 30 August 2019 by CeilidhWelsh (Talk | contribs)

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SP_lamB-PETase R280A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 540
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The entire amino acid sequence was codon optimised for E. coli by IDT’s on-line Codon Optimisation tool ensuring that there were no forbidden restriction sites, BsaI or SapI, to allow for TypeIIS assembly.

Source

Wild type amino acid sequence originates from Ideonella sakaiensis. Mutations have been directed by reference to the literature. Resulting DNA sequences were codon optimized by the IDT online software.

References