DNA
Part:BBa_K2927022
Designed by: KUO, PEI-CHING Group: iGEM19_CCU_Taiwan (2019-08-29)
Negative control sequence for crRNA 3 target
We changed 5 nucleotides of crRNA 1 from 5’ end to make the mutated crRNA 3. It is the sequence for testing whether homemade Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 3 is too short to become the functional protein. There is no concentration for spread out ASFV of our project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |