DNA

Part:BBa_K2927021

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-08-29)
Revision as of 14:33, 25 September 2019 by Peggy KUO (Talk | contribs)

Negative control sequence for crRNA 2 target

We have made a change on 5 nucleotides of crRNA 2 from 5’ end to make the mutated crRNA 2. It is the sequence for testing whether our purified Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 2 is too short to be the functional protein. Thus, there is no risk for the spread out of ASFV from our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None