Coding

Part:BBa_K2927002

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-08-28)
Revision as of 04:29, 29 August 2019 by Peggy KUO (Talk | contribs)

10X His

10 times histidine is made up of 10 histidines connected. Because of the affinity with Ni, it makes composition (10X His-MBP-TEV-Cas12a) bind on the Ni column. It’s used for Cas12a protein purification. The 10X histidine is used by E.coli.

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None