Coding

Part:BBa_K2973009:Design

Designed by: Nick Delkis, Aphrodite Katsaouni   Group: iGEM19_Thessaly   (2019-08-26)
Revision as of 22:15, 26 August 2019 by Nickdelkis (Talk | contribs) (References)


IS6110 (Mycobacterium tuberculosis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 880
    Illegal XhoI site found at 1266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 232
    Illegal NgoMIV site found at 1305
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1314
    Illegal BsaI.rc site found at 1295


Design Notes

NCBI Mycobacterium tuberculosis Genome, Strain H37Rv, Accession Number: NC_000962


Source

IS6110 has normally two open reading frames. OrfA (327 bp) and orfB (927 bp) are consecutive ORFs partially overlapping in the relative translational reading frames 0 and −1, respectively. For the purposes of our project, we designed primers for orfB only.

References

D Thierry, A Brisson-Noël, V Vincent-Lévy-Frébault, S Nguyen, J L Guesdon, B Gicquel, Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis (1990), Journal of Clinical Microbiology

Thabet, S., & Souissi, N. (2016). Transposition mechanism, molecular characterization and evolution of IS6110, the specific evolutionary marker of Mycobacterium tuberculosis complex. Molecular Biology Reports, 44(1), 25–34.doi:10.1007/s11033-016-4084-x