Composite

Part:BBa_K2973000:Design

Designed by: Nick Delkis   Group: iGEM19_Thessaly   (2019-08-17)
Revision as of 16:10, 17 August 2019 by Tkontogiannis (Talk | contribs) (→‎References)

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T7-RBS-β-Lactamase-No Signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 851
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Beta-lactamase needs to be truncated in its N-terminal end in order to work properly. Therefore, we deleted the signal peptide from its sequence in order to do our in vitro experiments.


Source

NCBI Reference Sequence: WP_000027057.1

References

Qureshi, Sohail A. “β-Lactamase: an Ideal Reporter System for Monitoring Gene Expression in Live Eukaryotic Cells.” BioTechniques, vol. 42, no. 1, 2007, pp. 91–96., doi:10.2144/000112292.

Boehle, Katherine E., et al. “Paper-Based Enzyme Competition Assay for Detecting Falsified β-Lactam Antibiotics.” ACS Sensors, vol. 3, no. 7, 2018, pp. 1299–1307., doi:10.1021/acssensors.8b00163.